Physical properties of oxidized phospholipid (OxPL) membranes consisting of binary
mixtures of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and 10 % of one of
two OxPLs - 1-palmitoyl-2-glutaryl-sn-glycero-3-phosphocholine (PGPC) or 1-palmitoyl-2-
(5'-oxo-valeroyl)-sn-glycero-3-phosphocholine (POVPC), were investigated experimentally
and computationally. Fluorescence solvent relaxation (SR) and fluorescence correlation
spectroscopy z-scan (FCS z-scan) show increased headgroup hydration and dynamics, and
faster lateral diffusion in POPC membrane upon addition of OxPLs. The magnitudes of both
effects are distinct for each of the two OxPLs. Molecular dynamics simulations corroborate
the experimental findings providing at the same time a detailed molecular interpretation in
terms of changes in phospholipid structure and orientation.